Dextran, high fraction, for biochemistry
Dextran
CAS: 9004-54-0
Molecular Formula: C17H32O10
Dextran, high fraction, for biochemistry - Names and Identifiers
Dextran, high fraction, for biochemistry - Physico-chemical Properties
| Molecular Formula | C17H32O10 |
| Molar Mass | 396.42998 |
| Melting Point | 483 °C (decomp) |
| Boling Point | -85.05ºC at 760 mm Hg |
| Specific Rotation(α) | 198 º |
| Water Solubility | Soluble in water, dimethyl sulfoxide, ethylene glycol and glycerol. |
| Solubility | Soluble in water. Slightly soluble in ethanol. |
| Appearance | White crystal or powder |
| Color | White to slightly off-white |
| Merck | 14,2948 |
| PH | 2 - 10 |
| Storage Condition | 2-8°C |
| Stability | Stable. Keep dry. Incompatible with strong oxidizing agents. |
| Sensitive | Easily absorbing moisture |
| Refractive Index | 185 ° (C=6, H2O) |
| MDL | MFCD00130935 |
| Physical and Chemical Properties | Iron Dextran Injection is a dark brown colloidal solution. The iron dextran solid was a dark tan amorphous powder. Odorless and astringent. Hygroscopic in the air. Soluble in water, the solution is dark brown colloidal solution, ph 5.2-6.5, insoluble in organic solvents such as ethanol. Iron Dextran Injection is a complex of iron and dextran. After intramuscular injection, part of the iron was phagocytosed by macrophages and transferred to fibroblasts, and the rest of the iron was slowly removed from the injected part (50% at 72h) and transferred to plasma via lymph. Three weeks after the injection, iron was still found in the blood. Iron was mainly stored in the reticuloendothelial cells of the liver and spleen and was slowly released for the need of hemoglobin synthesis. This absorption pathway is not controlled by the gastrointestinal tract, so the recovery of stored iron is rapid and complete. However, the rate of increase in hemoglobin is still the same as that of oral preparations, averaging about 0.15g per day. Effective for iron deficiency anemia. |
| Use | Medium molecule dextran is mainly used to increase plasma volume and maintain blood pressure, with anti Shock as the main. It is suitable for supplementing blood volume and maintaining blood pressure when there is a large amount of blood loss. Such as burns, trauma, trauma and other hemorrhagic and due to excessive blood loss caused by the body grams of first aid. Low molecular dextran, mainly to improve microcirculation, for the prevention or elimination of intravascular red blood cell aggregation and thrombosis. The action and use of small molecule dextran is similar to that of low molecular dextran. |
Dextran, high fraction, for biochemistry - Risk and Safety
| Risk Codes | R20/21/22 - Harmful by inhalation, in contact with skin and if swallowed. R36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S24/25 - Avoid contact with skin and eyes. S37/39 - Wear suitable gloves and eye/face protection S36 - Wear suitable protective clothing. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
| WGK Germany | 2 |
| RTECS | HH9230000 |
| FLUKA BRAND F CODES | 3 |
| TSCA | Yes |
| HS Code | 39139000 |
| Toxicity | LD50 oral in rat: > 3gm/kg |
Dextran, high fraction, for biochemistry - Reference
| Reference Show more | 1. Guo Ruixia, Li Min, Zhang Baohua, etc. Synthesis and characterization of genistein 4/'O-β-D-glucoside [J]. Coal Chemical Industry, 2013, 036(001):P.35-36,43. 2. Guo Ruixia, Feng Junxia, Jia Huizhen, etc. Study on synthesis of genistein -4'-O-glycoside [J]. Henan Chemical Industry, 2013, 030(007):36-38. 3. Yin Hua Wang Junjie, Si, Ji Qing, Wang, Ming, Li, Yue Duan, Jin Lian Duan, is steel. Experimental study on the main curative effect of four kinds of traditional Chinese medicine injection and the original solution [J]. Chinese Medicine Guide 2017 23(03):62-65. 4. Zhang, Wu, Zi, Yi, Zhao, Mo, na, et al. Experimental study on α-glucanase in sugar beet production [J]. Chinese sugar 2019 041(001):13-18. 5. Sun, Qing, Zhang, Shuming, Tan, Xiao et al. Screening and optimization of fermentation conditions of a high-yield glucan intestinal membrane subsp. Leuconostoc [J]. Food Science and Technology 2019(7). 6. Meng de You, Ji Yubin, the morning peak. Synthesis and physicochemical properties of laminarin sulfate with different degree of sulfur substitution [J]. Advances in modern biomedicine 2012 12(12):2298-2301. 7. Ji Yubin, Meng Deyou, Ji, morning peak. Synthesis and physicochemical properties of laminarin sulfate with different degree of sulfur substitution [J]. Journal of Jiangxi College of Traditional Chinese Medicine, 2011, 23(06):71-73. 8. Sun Liping, Li Xiao, Su Xuejiao. Properties and antioxidant activity of polysaccharides from Boletus Sinensis [J]. Science and Technology of Food Industry 2016 37(24):173-175 185. 9. Yue Pengfei, Tang Jianbin, Zheng Qin, Xu Guoliang, Yang Ming, Liu Ronghua. Evaluation of in vitro release behavior of compound Danshen sustained-release tablets based on the inhibition of neutrophil respiratory burst [J]. Chinese herbal medicine, 2010,41(06):885-888. 10. GE Yuxin, Zhang Yang, Tian Zhenhua, et al. Fluorescence Detection of (1-3)-β-D-glucan based on carbon dots [J]. Journal of Changchun University of Science and Technology (Natural Science Edition), 2017, 40(004):133-137. 11. Li Liang, Zhou Yan, Teng Fei, et al. Effect of jet cavitation on Maillard reaction and emulsification characteristics of soybean protein [J]. Journal of Agricultural Machinery, 2019, 050(008):372-378. 12. He Fei, Li Dongmei, Su Qi table, etc. Experimental study on the treatment of skin itching in mice with active components of the black and the Ant [J]. Chinese herbal medicine, 2018, 3 (5). 13. Ji Chenfeng, Meng Deyou, Ji Yubin. Preparation and anti-tumor activity of laminarin sulfate [J]. China Marine Medicine, 2013, 24 (02):1-6. 14. Yuan Rui, Wang Xuejiang, Li Feng. Extraction and molecular weight determination of sodium alginate from seaweed fertilizer [J]. Zhejiang Agricultural Sciences, 2019, 60(008):1275-1277. 15. He Wan-Ying, Li Xiao-yan, Sun Xiao-Wen, et al. Process optimization of dextran-soy protein isolate coupled grafting and physicochemical properties of graft product [J]. Science and Technology of food industry, 2018, 039(004):54-59. 16. You Juan, Jiang Xueying, Yin Tao, etc. Effects of dextran and its glycosylation modification on gel properties of silver carp surimi [J]. Chinese Journal of Food Science, 2019, v.19(12):75-81. 17. Guo Peixin, Liu Chengming. Effects of Xuesaitong Soft Capsule on microcirculation disturbance of eyeball in rabbits and Hemorheology in blood stasis rats [J]. Pharmacology and Clinic of Traditional Chinese Medicine, 2014, 030(001):58-61. 18. Chen Hualei, Hou dianzhi, Li Suxia, etc. Study on ultrasonic degradation of dextran and its kinetics [J]. Food Science and Technology, 2017(7):73-78. 19. Li Yi. Preparation, structure verification and characterization of superparamagnetic iron oxide (SPIO) and Polylysine (PLL) nanoparticles [J]. Fudan University Journal of Medical Sciences, 2014, 35 (41):309-314. 20. Li Lingyu, Li Yuchen, zuozhao, et al. Determination of degree of polymerization of inulin by high performance gel filtration chromatography [J]. Shandong Science, 2020, 26 (4):1-6. 21. Xu Yong, Zhang Ye. Effects of Ampelopsis grossedentata leaves on Hemorheology in rats with blood stasis [J]. Journal of Liaoning University of Traditional Chinese Medicine, 2016, 018(002):30-31. 22. Qiaoxin, Liu, Yang Jun, Cheng Kai, Huang yumong, Peng Ying. Encapsulation reaction and product characterization of two dihydrochalcone sweeteners [J]. China food additive 2019 30(03):51-58. 23. Lu Simin, He Xiaobing, Zhang Liting, Ding Hongyan, Qi Yi, Wu tie. Study on the effect of acid leaching high pressure method on the changes of three main components in Ganoderma lucidum spores [J]. Grain and Oil food science and technology, 2019,27(06):93-97. 24. Zhu, Xiao-Ping Xu, Ting Huang. Determination of total triterpenoids and polysaccharides in sporoderm-broken spores of Ganoderma lucidum by UV-Vis spectrophotometry [J]. Food safety guide, 2020(30):85 87. 25. Li Lingyu, Li Yuchen, zuozhao, Tian Baolan, Zheng Zhenjia. Determination of degree of polymerization of inulin by high performance gel filtration chromatography [J]. Shandong Science, 2020,33(04):1-6. 26. Xie Wenjing, Wang Jingjing, Liu Ying, Ding Zhou, Liu Ying. Study on fluorescence emission characteristics and mechanism of polysaccharide on white blood cells [J]. Spectroscopy and spectral analysis, 2021,41(04):1050-1054. 27. Huiyu Ma, Qilin Huang, Jiayi Ren, Zhaomin Zheng, Yidong Xiao, Structure characteristics, solution properties and morphology of oxidized yeast β-glucans derived from controlled TEMPO-mediated oxidation, Carbohydrate Polymers, Volume 250, 2020, 116924, ISSN 28. Jing, Hairong, et al. "Fission and Internal Fusion of Protocell with Membraneless “Organelles” Formed by Liquid-Liquid Phase Separation ." Langmuir 36.27 (2020): 8017-8026.https://doi.org/10.1021/acs.langmuir.0c01864 29. Liu, Hong, et al. "Evaluation of hydrogen yield potential from Chlorella by photo-fermentation under diverse substrate concentration and enzyme loading." Bioresource technology 303 (2020): 122956.https://doi.org/10.1016/j.biortech.2020.122956 30. Liu, Hong, et al. "Evaluation of hydrogen yield potential from Chlorella by photo-fermentation under diverse substrate concentration and enzyme loading." Bioresource technology 303 (2020): 122956.https://doi.org/10.1016/j.biortech.2020.122956 31. Chen, Hualei, et al. "Enzymatic degradation of aqueous dextrans as affected by initial molecular weight and concentration." Polymer Bulletin (2020): 1-14.https://doi.org/10.1007/s00289-020-03351-9 32. [IF=6.953] Yang Liu et al."Extraction optimization characterization, antioxidant and immunomodulatory activities of a novel polysaccharide from the wild mushroom Paxillus involutus."Int J Biol Macromol. 2018 Jun;112:326 33. [IF=9.147] Wentao Zhang et al."Profiling the water soluble pectin in clear red raspberry (Rubus idaeus L. cv. Heritage) juice: Impact of high hydrostatic pressure and high-temperature short-time processing on the pectin properties."Food Hydrocolloid. 2022 Apr;125:10 34. [IF=30.849] Mei Yang et al."Engineering Hibiscus-Like Riboflavin/ZIF-8 Microsphere Composites to Enhance Transepithelial Corneal Cross-Linking."ADVANCED MATERIALS. 2022 Apr 14 |
Dextran, high fraction, for biochemistry - Nature
Open Data Verified Data
This product is white powder. Odorless and tasteless. Soluble in hot water, insoluble in ethanol. Dextran is a dehydration polymer of several glucose molecules, also known as dextran, which widely exists in microorganisms in nature and is an important part of cell wall. Due to its large molecular weight, it cannot be directly used for medicine, it must be hydrolyzed into molecules of a certain size. The molecular weight of 25000~50000 is low molecule, 50000~0000 is medium molecule. This product is a low molecular dextran, mainly used for blood volume expansion agent, the aqueous solution of colloidal solution, can maintain good colloid osmotic pressure. Easily excreted from the kidney, half-life of only 3 hours. The osmotic pressure of 10% solution is slightly higher than that of plasma protein, which can absorb the liquid in the tissue into the blood, so it can dilute the blood quickly, reduce the hematocrit and plasma viscosity, accelerate the blood flow, and dredge the microcirculation, it can reduce the aggregation of red blood cells in blood vessels and platelet aggregation. Also hinder the platelet release of the third factor, a mild anticoagulant effect.
Last Update：2024-01-02 23:10:35
Dextran, high fraction, for biochemistry - Standard
Authoritative Data Verified Data
high molecular weight glucose polymer produced by sucrose of this strain after fermentation by Leuconostoc Leuconostoc mesenteroides No. L.-M-1226, was obtained by treatment and purification. The weight average molecular weight (Mw) of dextran 20 should be from 16,000 to 24,000.
Last Update：2024-01-02 23:10:35
Dextran, high fraction, for biochemistry - Trait
Authoritative Data Verified Data
- This product is white powder; Odorless.
- This product is soluble in hot water and insoluble in ethanol.
specific rotation
take this product, precision weighing, add water to dissolve and quantitatively dilute to make a solution containing about 10 mg per lml, at 25°C, according to the law (General 0621), the specific rotation was 190 ° to 200 °.
Last Update：2022-01-01 11:36:18
Dextran, high fraction, for biochemistry - Use
Open Data Verified Data
blood volume supplement.
Last Update：2022-01-01 11:13:28
Dextran, high fraction, for biochemistry - Differential diagnosis
Authoritative Data Verified Data
take 0.2g of this product, add 5ml of water to dissolve, add 2ml of sodium hydroxide test solution and several drops of copper sulfate test solution to generate light blue precipitate, and turn brown precipitate after heating.
Last Update：2022-01-01 11:36:19
Dextran, high fraction, for biochemistry - Safety
Open Data Verified Data
stored in a dry place.
Last Update：2022-01-01 11:13:29
Dextran, high fraction, for biochemistry - Exam
Authoritative Data Verified Data
molecular weight and molecular weight distribution
take an appropriate amount of this product, add the mobile phase to dissolve and dilute to make a solution containing about 10 mg per lml, shake, and place overnight as the test solution; in addition, 4 to 5 dextran reference products of known molecular weight were taken, and a solution containing 10 mg per 1 ml was prepared as a reference solution by the same method. According to the molecular exclusion chromatography method (General 0514), the hydrophilic spherical polymer is used as the filler (TSKGPWXL column, ShodexOHpakSBHQ column or other suitable column); The mobile phase is 0.71% sodium sulfate solution (containing 0.02% sodium azide); column temperature was 35°C; Flow rate was 0.5ml per minute; Differential refractive index detector.
weigh glucose and dextran
- 2000 in proper amount, add mobile phase separately to dissolve and dilute to prepare a solution containing about 10 mg per 1 ml. Inject the liquid chromatograph and measure the retention time tr and, the retention time tR of the main peak in the chromatogram of the test solution and the control solution should be between tr and. The number of theoretical plates is not less than 5000 in terms of the glucose peak.
- the above reference solution 20 u1 was injected into human liquid chromatograph respectively, the chromatogram was recorded, and the regression equation was calculated by GPC software. The sample solution 20 u1 was taken and measured by the same method, and the weight average molecular weight and molecular weight distribution of the sample were calculated by GPC software. The weight average molecular weight of 10% macromolecule part of this product shall not be more than 70 000, and the weight average molecular weight of 3500 small molecule part shall not be less.
chloride
take 0.10g of this product, add 50ml of water, heat and dissolve, cool, take 10ml of solution, check according to law (General rule 0801), and compare with the control solution made of 5ml of standard sodium chloride solution, no more concentrated (0.25%).
ammonia
take 0.20g of this product, put it in a 50ml Kjeldahl Flask, add 1ml of sulfuric acid, heat and digest until the test product is black oily, let it cool, add 2ml of 30% hydrogen peroxide solution, heat and digest until the solution is clear (if it is not clear, add 0.5~1.0 of the above hydrogen peroxide solution and continue heating), cool to below 20°C, Add 10ml of water, add 5% sodium hydroxide solution Dropwise to make it alkaline, move it to 50ml Cuvette, wash the flask with water, wash the flask with human Cuvette, dilute it with water until the scale, slowly add 2ml alkaline mercuric iodide test solution, shake with the addition (the temperature of the solution is kept below 20°C); In case of color development, mix with standard ammonium sulfate solution (precision weighing 105g of ammonium sulfate dried at 0.4715°C to constant weight), in a 100ml measuring flask, water was added to dissolve and dilute to the scale, mixed well, and used as a stock solution. At the time of use, take 1ml stock solution accurately, put it in a 100ml measuring flask, dilute it with water to the scale, and shake it well. Each 1ml equivalent to the lOug of N) 0.007% ml plus sulfuric acid after treatment with the same method of color comparison, not deeper ().
loss on drying
take this product, dry at 105°C for 6 hours, loss of weight shall not exceed 5.0% (General rule 0831).
ignition residue
take 1.5g of this product and check it according to law (General rule 0841). The remaining residue shall not exceed 0.5%.
Heavy metals
The residue left under the ignition residue item shall not contain more than 8 parts per million of heavy metals after examination by law (General Principles 0821, Law II).
Last Update：2022-01-01 11:36:20
Dextran, high fraction, for biochemistry - Category
Authoritative Data Verified Data
Plasma substitutes.
Last Update：2022-01-01 11:36:20
Dextran, high fraction, for biochemistry - Storage
Authoritative Data Verified Data
sealed and stored in a dry place.
Last Update：2022-01-01 11:36:21
Dextran, high fraction, for biochemistry - Dextran 20 and Glucose Injecrion
Authoritative Data Verified Data
This product is a sterile aqueous solution of dextran 20 and glucose. Dextran 20 and glucose (C6H1206 • H20) should be 95 of the labeled amount. 0 ~ 105.0%.
trait
This product is colorless, slightly viscous clear liquid, sometimes slight opalescence.
identification
- take 1ml of this product, add 2ml of sodium hydroxide test solution and several drops of copper sulfate test solution to generate light blue precipitate, which turns to brown precipitate after heating.
- take 1ml of this product and slowly drop it into the warm alkaline copper tartrate test solution to generate red precipitate of cuprous oxide.
examination
- molecular weight and molecular weight distribution the weight average molecular weight of the product shall be 16 000 to 24 000, and the weight average molecular weight of the 10% macromolecular fraction shall not be greater than 70000, as determined by dextran 20, 10% the weight average molecular weight of small molecules shall not be less than 3500.
- the pH value should be 3.5 to 6.5 (General 0631).
- 5-hydroxymethyl Bran precision measure an appropriate amount of this product (about 1.0g equivalent to glucose), put it in a 100ml measuring flask, dilute it with water until it is calibrated, and shake it well, according to UV-visible spectrophotometry (General rule 0401), measured at the wavelength of 284nm, the absorbance should not be greater than 0.32.
- Heavy Metal Precision take 20ml of this product, place it in a crucible, steam dry, and check according to law (General rule 0821 second law), containing no more than of heavy metals.
- The osmolality should be 265 to 325 mosmol/kg (General 0632).
- abnormal toxicity of this product, according to law inspection (General 1141), should comply with the provisions.
- bacterial endotoxin this product, according to the law to check (General 1143), the amount of endotoxin per lml should be less than 0.50EU.
- allergic reaction take this product, according to the law inspection (General 1147), should comply with the provisions.
- others should comply with the relevant provisions under injection (General 0102).
Content determination
glucose precision take this product 2ml, put the iodine bottle, precision Iodine titration solution (0.05mol/L)25ml, while shaking the drop of sodium hydroxide titration solution (0.lmol/L )50ml, placed in the dark for 30 minutes, plus dilute sulfuric acid 5ml, with sodium thiosulfate titration solution (0.lmol/L) titration, when the end point is near, add 2ml of starch indicator solution, continue titration until the blue color disappears, and use 0.12g(6% specification) or 0.20g (10% specification) of dextran 20 for blank test correction. This corresponds to 9.909mg of C6H1206 • H2O per 1 ml of iodine titrant (0.05mol/L).
category
with dextran 20.
specification
(1) 10% Dextran 20 and Glucose Injecrion ML: 10g dextran 20 with 5g glucose; 250ml:25g dextran 20 with 12.5g glucose; 500ml:50g dextran 20 with 25g glucose
(2) 6% Dextran 20 and Glucose Injecrion 100ml:6g dextran 20 with 5g glucose; 250ml:15g dextran 20 with 12.5g glucose; 500ml:30g dextran 20 with 25g glucose
storage
sealed storage at 25°C or less.
Last Update：2022-01-01 11:36:22
Dextran, high fraction, for biochemistry - Dextran 20 and sodium chloride injection
Authoritative Data Verified Data
This product is a sterile aqueous solution of dextran 20 and sodium chloride. Dextran 20 and sodium chloride (NaCl) should be 95.0% to 105.0% of the label amount.
trait
This product is colorless, slightly viscous clear liquid, sometimes slight opalescence.
identification
- the same reaction was shown in the Identification Test (1) under dextran 20 glucose injection.
- This product shows the reaction of sodium salt identification (1) and chloride identification (1) (General rule 0301).
examination
- molecular weight and molecular weight distribution the weight average molecular weight of the product shall be 16 000 to 24 000, and the weight average molecular weight of the 10% macromolecular fraction shall not be greater than 70000, as determined by dextran 20, 10% the weight average molecular weight of small molecules shall not be less than 3500.
- the pH value should be 4.0 to 7.0 (General 0631).
- osmolality should be 265 to 325mOsmol/kg (General 0632).
- heavy metals, abnormal toxicity, bacterial endotoxin and allergic reaction should be checked according to the method of dextran 20 glucose injection.
- others should comply with the relevant provisions under injection (General 0102).
Content determination
- dextran 20 take 10ml of this product, put it in a 25ml(6% specification) or 50ml(10% specification) measuring flask, dilute it with water to the scale, and shake it well, photometry (General 0621).
- take 10ml of sodium chloride, put it in an Erlenmeyer flask, add several drops of potassium chromate indicator solution, and titrate with silver nitrate titration solution (0.lmol/L). Each 1 ml of silver nitrate titration solution (0.1 mol/L) corresponds to 5.844mg of NaCl.
category
with dextran 20.
specification
- 10% dextran 20 sodium chloride injection 100ml:10g dextran 20 with 0.9g sodium chloride; 250ml:25g dextran 20 with 2.25g sodium chloride; 500ml:50g dextran 20 with 4.5g sodium chloride
- 6% dextran 20 sodium chloride injection 100ml:6g dextran 20 with 0.9g sodium chloride; 250ml:15g dextran 20 with 2.25g sodium chloride; 500ml:30g dextran 20 with 4.5g sodium chloride
storage
sealed storage at 25°C or less.
Last Update：2022-01-01 11:36:22
Dextran, high fraction, for biochemistry - Reference Information
| EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
| What is glucan | glucan (glucan), it is found in the mucus secreted by some microorganisms during the growth process, which is divided into α-glucan and β-glucan, later, Dr. Diluzio of the University of Tulun isolated the resistant substance in baker's yeast and identified it as β-glucan. Β-glucan is widely found in yeast, mushrooms, oats and barley and other foods, of which β- 1,3 / 1,6 exists in yeast and mushroom glucan, and β- 1, 3/1, 4 is present in oat and barley glucans. Beta-glucan is different from common sugars (such as starch, glycogen, dextrin, etc.), the main difference is that the Bond connection is not the same, the molecular structure of common sugars is alpha -1,4-glycosidic bond, in contrast, β-glucan is mainly composed of β-1, 3-glycosidic bonds and contains some β-1, 6-glycosidic bonds. Β-glucan has a spiral-shaped molecular structure due to its special Bond connection and the presence of intramolecular hydrogen bonds. This unique configuration is easily accepted by the immune system. |
| History of dextran | in the twentieth century, for the first time, Dr. Pillemer has discovered and reported that a substance in the cell wall of yeast has an immune-boosting effect. Later, after further research by Dr. Diluzio of the University of Tulun, it was found that the substance that improves the immunity of the yeast cell wall is a kind of polysaccharide-beta-glucan, which is isolated from baker's yeast. |
| pharmacological action | the average molecular weight of dextran is about 7000, which is similar to human albumin and can improve plasma colloid osmotic pressure, absorb the water outside the blood vessel to supplement blood volume, so as to maintain blood pressure; Can make the aggregation of red blood cells and platelets, reduce blood viscosity, thereby improving microcirculation and tissue perfusion, prevent intravascular coagulation in the late Shock; Can inhibit the activation of coagulation factor II, so that the activity of coagulation factor I and coagulation factor VIII decreased, which and its antiplatelet effect can prevent thrombosis. The blood volume expansion effect and antithrombotic effect of dextran were stronger than that of dextran -40. |
| Use | anti-anemia drug. For iron deficiency anemia. Adverse reactions and contraindications after injection of this product hemoglobin did not gradually increase, should be discontinued. Liver, renal insufficiency should not be used. blood volume supplement. Adverse reactions and contraindications occasional allergic reactions. Congestive heart failure and hemorrhagic disease patients disabled, heart, liver, renal insufficiency with caution. Middle molecular dextran is mainly used to increase plasma volume and maintain blood pressure, and is mainly used in anti Shock. It is suitable for supplementing blood volume and maintaining blood pressure when there is a large amount of blood loss. Such as burns, trauma, trauma and other hemorrhagic and due to excessive blood loss caused by the body grams of first aid. Low molecular dextran, mainly to improve microcirculation, for the prevention or elimination of intravascular red blood cell aggregation and thrombosis. The action and use of small molecule dextran is similar to that of low molecular dextran. Biochemical Study |
| production method | sucrose was used as culture medium, which was obtained by fermentation and purification of Leuconostoc sp. microbial fermentation method for strain breeding of intestinal membrane-like Leuconostoc (Leuconostoc mesetoides). Preparation of medium: according to the ratio of mass/volume, sucrose 10%, peptone 0.25%, disodium hydrogen phosphate 0.08%, add water to 100%, boil and dissolve, filter paper, take 3ml of filtrate into a test tube, plug with gauze cotton, place into the grid, sterilize under pressure at 117.72kPa(1.2kgf/cm2) 120 ℃ for 30min, cool, and put into an incubator to obtain clear and transparent liquid medium for inoculation. According to the above liquid medium ratio, add 1.5%-2% agar, boil and dissolve, Take 5ml sub-packed in a test tube, plug with gauze cotton, 117.72 kPa (1.2kgf/cm2), pressure sterilization 30min, cooling, that is, the solid medium. Purification and culture of the strain 5 solid medium were taken, heated and melted, and then incubated in a water bath at 50-60 ℃, and numbered respectively. Select the culture tube in the sterile cabinet, dip one ear with platinum ear and inoculate it in No. 1 test tube with solid medium, shake well, then dip the No. 1 test tube and inoculate it in No. 2 test tube, shake well and inoculate to No. 5 test tube successively. Then pour it into the Petri dish of the corresponding number by tube while hot (about 50 ° C), level it, cool it, and place it in an incubator, and incubate it at 25 ° C for 24 hours, regular edges, slightly convex in middle, transparent, sticky colony. In the No. 3-5 petri dish selection of bacteria, the normal number of colonies should be 5-20 shall prevail. Circle the outer wall of the Petri dish with crayons, and select typical colonies (with obvious characteristics and appropriate size). The colonies delineated by Platinum ear dipping were inoculated in a tube containing liquid medium in a sterile cabinet and incubated at 25 ° C. For 24 hours. After 2-3 generations, the small sample fermentation, hydrolysis, division and other tests are carried out, and the strains with high yield, good composition and high yield are selected, and the refrigerator is kept at 2-4 ℃ for expanded production and use. Intestinal membrane-like Leuconostoc [culture medium] →[25 ℃,24h] colony seed culture liquid culture medium 400ml (medium bottle) and 4000ml (large bottle), one strain test tube (3ml) inoculation was carried out in a medium bottle, and about 100ml of the seed solution cultured in the medium bottle was inoculated in a large bottle. At 25 ℃ 20-24H, end pH3.8-4.3, good fermentation can be used for production. Colony [liquid medium] →[25 ℃, 20-24h] Seed culture solution fermentation, precipitation according to sucrose 15%, peptone 0.25%, disodium hydrogen phosphate 0.15%, add normal water to 100%, the fermentation culture medium is prepared in the fermentor, the inoculation amount is 2.5%, the stirring is 10-15min, the control is pH7-7.4,25 ℃ or so, the fermentation is 20-24H, the final fermentation liquid pH is 4.2-5, reaches the end point. 85%± 5% ethanol was added to precipitate, and the precipitate was washed with 60%-70% ethanol to obtain crude high molecular weight dextran for hydrolysis in 85% yield. Seed culture medium [fermentation medium] →[25 ℃,20-24H, pH7-7.4] fermentation broth [85% ethanol, 60%-70% ethanol] →[pH4.2-5] high molecular weight dextran crude product hydrolysis, neutralization, purification the crude polymer Dextran was heated and dissolved with distilled water, calculated according to the mass of the hydrolysate, 0.1% hydrochloric acid was added, kept at 95-100 ℃, hydrolyzed, and diluted with distilled water to a concentration of 11%, control the end viscosity of 2.7-2.9, with 6mol/L NaOH slowly neutralized to pH6-6.5, add anhydrous calcium chloride 2.4g/L (0.24%), finally, 8g/L (766) of type 0.8% coarse-grained activated carbon was added, decolorized with stirring, and filtered to obtain a Hydrolysate for division. The crude product [HCl, NaOH, CaCl 2, 766 activated carbon] →[95-100 ℃, pH6-6.5] is divided into the first-order division and the hydrolysis solution is added with ethanol to make the concentration of 40%-40.5%, after keeping the temperature at 40 ℃ for 22h, the supernatant was collected and divided into two stages. The precipitates were impurities and macromolecular dextran. The supernatant after two stage Division and one stage Division was added with ethanol to make its concentration reach 45%-45.5%, stirred for 15min, kept at 40 ℃ for 22h, and the precipitate was medium molecular dextran. The supernatant after three-stage Division and two-stage Division was added with ethanol to make its concentration up to 48%-48.5%, stirred for 15min, kept at 40 ℃ for 22h, and precipitated as low molecular dextran. Four levels of three levels of the supernatant after the division of the ethanol, the concentration of 55.5% a 56.5%, stirring 15min, room temperature static 6h, precipitation of small molecular dextran. The obtained Dextran was divided by drying, dehydrated by blending powder with more than 90% ethanol and removing impurities, and made into loose alcohol-containing powder, which was centrifuged and dried to obtain dextran finished product with an overall yield of 60%. Dextran finished product [more than 90% ethanol] → powder containing alcohol [centrifuge, dry] → preparation of dextran finished injection the finished dextran and glucose or sodium chloride respectively are prepared into sterilized aqueous solution as required for injection. Dextrose finished product [glucose, water] → glucose injection dextran finished product [sodium chloride, water] → [sterile] Sodium chloride injection. raw material treatment the Dextran was used to separate the liquid, and the relative molecular mass of 5000-7500 was recovered as raw material. Decolorizing and mixing about 300ml of water was added to the container, and after heating and boiling, 100g of dextran was added and stirred until all of them were dissolved, and additional water was added to prepare 100g/L. Add 30g/L(3%) activated carbon according to the mass ratio of dextran, stir, boil for 15-30 min, filter with No. 3 vertical melting funnel to obtain clear filtrate, and let it cool to room temperature. Take the filtrate to a suitable container, add 65% g/L () ferric chloride solution with stirring, and stir well to obtain a mixture. Dextrose [distilled water, activated carbon] → [boiling] filtrate [FeCl3]→ The mixed solution was catalyzed by 701 × glass ion exchange column, loaded with styrene type weak basic anion exchange resin, with 100g/L (10%)NaOH as regenerant, the resin is converted into anion OH-type, the effluent is pH7-8, no chloride, and the specific resistance is above 50 kΩ/cm, which is used for catalytic reaction. The water level in the regenerated resin column is placed at the interface of the resin, and the mixture is poured into the dynamic catalytic reaction. It is advisable to control the outflow rate at 15ml/min. When the deep red iron dextran flows out, immediately take a sample to check the pH value, chloride and specific resistance, the pH should be above 8, Chloride should be negative, specific resistance above 2 kΩ/cm, collected after passing. When the mixed solution drops to the interface of the resin, it starts to be eluted with water, and the inlet velocity is controlled at the same as the outflow velocity, and the effluent can generally be collected at 2-3L. Mixed Liquor [701 resin column] → effluent (Iron Dextran) concentrate the above effluent for concentration under reduced pressure, temperature between 45-65 ℃, stop distillation when 1L, take sample to measure iron content and adjust iron content as appropriate, make it to 2.5%. Effluent [40-65 ℃, reduced pressure] → concentrated liquid preparation the prepared solution is filtered through No. 3 or No. 4 vertical melting glass funnel, operated according to sterilized preparation, subpackaged, sealed, 112 ℃,30min sterilization, that is, iron dextran injection products. Concentrated solution [112 ℃,30min]→ Iron Dextran Injection product. |
Last Update：2024-04-09 20:52:54
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